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KMID : 0624620200530070357
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BMB Reports
2020 Volume.53 No. 7 p.357 ~ p.366
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Recent advances in intravital microscopy for investigation of dynamic cellular behavior in vivo
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Choo Yeon-Woong
Jeong Ju-Hee
Jung Kee-Hoon
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Abstract
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Currently, most biological research relies on conventional experimental techniques that allow only static analyses at certain time points in vitro or ex vivo. However, if one could visualize cellular dynamics in living organisms, that would provide a unique opportunity to study key biological phenomena in vivo. Intravital microscopy (IVM) encompasses diverse optical systems for direct viewing of objects, including biological structures and individual cells in live animals. With the current development of devices and techniques, IVM addresses important questions in various fields of biological and biomedical sciences. In this mini-review, we provide a general introduction to IVM and examples of recent applications in the field of immunology, oncology, and vascular biology. We also introduce an advanced type of IVM, dubbed real-time IVM, equipped with video-rate resonant scanning. Since the realtime IVM can render cellular dynamics with high temporal resolution in vivo, it allows visualization and analysis of rapid biological processes.
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KEYWORD
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Cell biology, Cell trafficking, Cellular behavior, In vivo imaging, Intravital microscopy
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